4TL8

Crystal structure of N-terminal C1 domain of KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-23
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	79.934, 133.215, 150.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.591 - 1.859
R-factor	0.1826
Rwork	0.180
R-free	0.22200
Starting model (for MR)	2gbl
RMSD bond length	0.014
RMSD bond angle	1.486
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.520
High resolution limit [Å]	1.859
Number of reflections	132647
<I/σ(I)>	48
Completeness [%]	97.8
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	PEG 400 or PEG 8000