4TK0

Crystal Structure of human Tankyrase 2 in complex with DPQ.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	74.040, 79.570, 153.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.820 - 1.650
R-factor	0.1878
Rwork	0.187
R-free	0.22500
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS (2005/3)
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.570	79.570	1.670
High resolution limit [Å]	1.650	4.710	1.650
Rmerge	0.062	0.029	0.602
Total number of observations	709713
Number of reflections	101327	5079	1416
<I/σ(I)>	18.45	55.03	1.81
Completeness [%]	92.4	99.5	77.6
Redundancy	6.47	6.78	3.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M NaCl, 0.1M Sodium, Hepes buffer at pH7.5, 12-15% iso-propanol