4S2Y

Structure of E. coli RppH bound to RNA and three magnesium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	79.631, 36.484, 58.073
Unit cell angles	90.00, 102.30, 90.00

Refinement procedure

Resolution	19.451 - 1.600
R-factor	0.1735
Rwork	0.172
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4s2w
RMSD bond length	0.007
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.050	0.643
Number of reflections	21065
<I/σ(I)>		2.71
Completeness [%]	96.7	90.6
Redundancy	5.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	crystal growth: 0.4 M ammonium sulfate, 12% PEG 3350, 10% glycerol. crystal soaking and cross-linking: 0.02 M sodium acetate pH 5.0, 0.2 sodium sulfate, 15% PEG 3350, 15% glycerol. crystal soaking and cryoprotection: 0.05 M MOPS-Na pH 7.0, 0.025 M magnesium chloride, 15% PEG 3350, 25% pentaerythritol propoxylate 5/4 PO/OH, VAPOR DIFFUSION, HANGING DROP, temperature 295K