4S2C
Covalent complex of E. coli transaldolase TalB with fructose-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91914 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.656, 91.152, 130.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.430 - 2.200 |
| R-factor | 0.15586 |
| Rwork | 0.153 |
| R-free | 0.21103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ucw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.430 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.094 | 0.491 |
| Number of reflections | 41815 | |
| <I/σ(I)> | 12.7 | 3.7 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.2 | 0.2 M DL-malic acid, pH 7.0, 19% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |
