4S0N
Crystal Structure of HLTF HIRAN Domain bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-10-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.026, 74.211, 66.180 |
| Unit cell angles | 90.00, 113.68, 90.00 |
Refinement procedure
| Resolution | 34.721 - 1.501 |
| R-factor | 0.1544 |
| Rwork | 0.153 |
| R-free | 0.18490 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.973 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 86121 | |
| <I/σ(I)> | 27.2 | 3.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289.15 | 25% PEG 4000, 250 mM sodium acetate trihydrate, 100 mM Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289.15K |
