4RZ6
Transaldolase B E96Q F178Y from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.844, 89.513, 130.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.070 - 1.802 |
| R-factor | 0.20322 |
| Rwork | 0.201 |
| R-free | 0.24907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onr monomer |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.070 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.700 |
| Number of reflections | 73150 | |
| <I/σ(I)> | 8.2 | 1.6 |
| Completeness [%] | 98.8 | 95.6 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.2 | 0.15M (NH4)2SO4, 18% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |
