4RYF

ClpP1/2 heterocomplex from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-19
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	97.230, 127.150, 265.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.18144
Rwork	0.180
R-free	0.21573
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jcq 4jct
RMSD bond length	0.004
RMSD bond angle	0.919
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.096	0.480
Number of reflections	78792
<I/σ(I)>	14.2	3.5
Completeness [%]	96.2	98.1
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M Sodium-Malonate, 40% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K