4RY5

C-terminal mutant (W550N) of HCV/J4 RNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-11
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	105.490, 107.946, 133.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.710
R-factor	0.1711
Rwork	0.166
R-free	0.24850
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nb4
RMSD bond length	0.009
RMSD bond angle	1.270
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.880	2.760
High resolution limit [Å]	2.710	2.710
Rmerge		0.460
Number of reflections	42052
<I/σ(I)>	12.2	3.7
Completeness [%]	96.6	94
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	50 mM MES, pH 5.0, 20% PEG4000, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K