4RWP

Crystal structure of porcine OAS1 in complex with dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 43 21 2
Unit cell lengths	72.668, 72.668, 189.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.680 - 2.250
R-factor	0.195
Rwork	0.194
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.813
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.680
High resolution limit [Å]	2.250
Number of reflections	25074
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	100 mM KCl, 100 mM HEPES pH 7.0, 15% PEG 5000 MME, VAPOR DIFFUSION, SITTING DROP, temperature 291K