4RWN

Crystal structure of the pre-reactive state of porcine OAS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	P 43 21 2
Unit cell lengths	72.400, 72.400, 205.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.040 - 2.000
R-factor	0.19
Rwork	0.188
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.040
High resolution limit [Å]	2.000
Number of reflections	37965
Completeness [%]	99.5
Redundancy	6.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	291	50 mM Tris-HCl pH 8.3, 100 mM KCl, 10 mM MgCl2, 23% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 291K