4RWI
Crystal structure of V561M FGFR1 gatekeeper mutation (C488A, C584S, V561M), apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.061, 50.550, 66.529 |
| Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
| Resolution | 47.499 - 2.292 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.23620 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ky2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.133 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
| High resolution limit [Å] | 2.290 | 6.210 | 2.290 |
| Rmerge | 0.030 | 0.177 | |
| Number of reflections | 30355 | ||
| <I/σ(I)> | 36.3 | ||
| Completeness [%] | 99.7 | 99.3 | 96 |
| Redundancy | 3.7 | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 0.1 M sodium cacodylate pH 6.4, 30% PEG 8000, 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
