4RSV
Human Obscurin Ig58 Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2014-04-10 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 44.482, 44.482, 177.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.640 | 2.620 |
High resolution limit [Å] | 2.411 | 2.560 |
Rmerge | 0.033 | 0.317 |
Number of reflections | 4329 | |
<I/σ(I)> | 29.47 | 3.08 |
Completeness [%] | 95.8 | 69.2 |
Redundancy | 3.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 0.3-0.5M NaCl 19-23% PEG 3350 10.52-11.35 mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |