4RSQ
2.9A resolution structure of SRPN2 (K198C/E359C) from Anopheles gambiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.925, 164.392, 186.181 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.900 |
| R-factor | 0.1968 |
| Rwork | 0.194 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pzf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.820 | 47.820 | 2.950 |
| High resolution limit [Å] | 2.900 | 15.880 | 2.900 |
| Rmerge | 0.135 | 0.045 | 0.635 |
| Rpim | 0.032 | 0.418 | |
| Total number of observations | 2507 | 20876 | |
| Number of reflections | 129666 | ||
| <I/σ(I)> | 7.9 | 18.6 | 2 |
| Completeness [%] | 99.5 | 93.4 | 99.8 |
| Redundancy | 3.4 | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.9 | 293 | 1.0 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic, pH 6.9, vapor diffusion, temperature 293K |
