4RP5
Crystal Structure of the L27 domain of Discs Large 1 (target ID NYSGRC-010766) from Drosophila melanogaster (space group P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.546, 47.818, 64.424 |
| Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
| Resolution | 27.016 - 1.650 |
| R-factor | 0.1835 |
| Rwork | 0.182 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rp3 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 4.470 | 1.650 |
| Rmerge | 0.047 | 0.031 | 0.544 |
| Number of reflections | 20902 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 99.7 | 96.8 | 99.8 |
| Redundancy | 4.1 | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0.2 M Sodium Format, 20 % PEG 3350, 3 % 1,5-Diaminopentane dihydrochloride, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
