4RN5

B1 domain of human Neuropilin-1 with acetate ion in a ligand-binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-12-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	62.839, 62.839, 87.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.540 - 1.730
R-factor	0.1776
Rwork	0.176
R-free	0.20080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.384
Data reduction software	d*TREK
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.540	1.840
High resolution limit [Å]	1.730	1.730
Number of reflections	16198
Completeness [%]	84.9	31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	100mM MES, 200mM Zinc Acetate, 10% PEG 8000, pH 6.0, VAPOR DIFFUSION, temperature 293K