4RMC

Crystal Structure of human retinoid X receptor alpha-ligand binding domain complex with 9cUAB76 and the coactivator peptide GRIP-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-02-16
Detector	RIGAKU
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	65.846, 65.846, 112.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.187
Rwork	0.187
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oap
RMSD bond length	1.357
RMSD bond angle	2.364
Data reduction software	DENZO
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.250	2.070
High resolution limit [Å]	2.000	2.150	2.000
Number of reflections	81222
Completeness [%]	98.5	96.6	93.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	5-15% PEG4000, 4-12% Glycerol, 0.1M Bis-Tris, pH=7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K