4RH5
Crystal structure of PTPN3 (PTPH1) in complex with Eps15 pTyr849 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.958, 67.500, 69.621 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.872 - 1.600 |
R-factor | 0.1752 |
Rwork | 0.174 |
R-free | 0.20350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b49 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.423 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.060 | 0.360 |
Number of reflections | 36684 | |
<I/σ(I)> | 31.8 | 4.8 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 7.6 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 21% PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |