4RFN
Crystal structure of ADCC-potent Rhesus macaque ANTIBODY JR4 in complex with HIV-1 CLADE A/E GP120 and M48
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 110.255, 77.827, 127.587 |
| Unit cell angles | 90.00, 114.26, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.210 |
| R-factor | 0.27623 |
| Rwork | 0.273 |
| R-free | 0.33226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4RFE for JR4 PDB entry 4H8W for gp120 PDB entry 2I5Y for M48 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.148 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.230 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.252 | 0.871 |
| Number of reflections | 33553 | |
| <I/σ(I)> | 6.9 | 1.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
