4REC
A nuclease-DNA complex form 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.910, 109.600, 115.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.600 - 2.200 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | an initial model phased by a low-resolution iodine-anomalous dataset |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.600 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | |
| Number of reflections | 89740 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-batch under oil | 300 | 0.1M potassium iodide, 0.1mM spermidine, 18% PEG3350, 0.1M BisTris propane, micro-batch under oil, temperature 300K |
