4RE1

Crystal structure of human TEAD1 and disulfide-engineered YAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	44.250, 105.030, 164.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.570 - 2.200
R-factor	0.2137
Rwork	0.212
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kys
RMSD bond length	0.009
RMSD bond angle	1.114
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.570	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.533
Number of reflections	38411
<I/σ(I)>		2.2
Completeness [%]	96.8	98.3
Redundancy		3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	0.1M sodium acetate, 1.8M sodium formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K