4RCP
Crystal structure of Plk1 Polo-box domain in complex with PL-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.390, 61.270, 51.260 |
| Unit cell angles | 90.00, 103.27, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| Rwork | 0.189 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.226 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.071 | 0.045 | 0.365 |
| Number of reflections | 30636 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 98.4 | 99 | 85.6 |
| Redundancy | 3.2 | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 293 | 0.2M sodium citrate pH 4.0, 500mM ammonium sulfate, vapor diffusion, temperature 293K |
