4RCJ
Crystal structure of YTHDF1 YTH domain in complex with 5mer m6A RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791829 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.648, 40.029, 59.308 |
| Unit cell angles | 90.00, 93.41, 90.00 |
Refinement procedure
| Resolution | 40.030 - 1.600 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rci |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.944 |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.030 | 40.030 | 1.630 |
| High resolution limit [Å] | 1.600 | 8.790 | 1.600 |
| Rmerge | 0.102 | 0.035 | 0.609 |
| Total number of observations | 505 | 3203 | |
| Number of reflections | 23138 | ||
| <I/σ(I)> | 9.4 | 21 | 1.8 |
| Completeness [%] | 99.4 | 97.5 | 93.5 |
| Redundancy | 3.6 | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG-3350, 0.2 M sodium chloride, 0.1 M bis-tris, pH 6.5, vapor diffusion, sitting drop, temperature 293K |
