4RAL
Crystal structure of insulin degrading enzyme in complex with macrophage inflammatory protein 1 beta
Replaces: 4QLDExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-08 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 65 |
Unit cell lengths | 263.117, 263.117, 90.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.263 - 3.148 |
R-factor | 0.1918 |
Rwork | 0.191 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cww |
RMSD bond length | 0.004 |
RMSD bond angle | 0.848 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.263 | |
High resolution limit [Å] | 3.148 | 3.148 |
Rmerge | 0.209 | |
Number of reflections | 59484 | |
<I/σ(I)> | 7.4 | |
Completeness [%] | 95.5 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 13% PEG5000 MME, 100 mM HEPES, pH 7.0, 10% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |