4R82
Streptomyces globisporus C-1027 NADH:FAD oxidoreductase SgcE6 in complex with NAD and FAD fragments
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97935 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.046, 62.830, 70.089 |
Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
Resolution | 34.928 - 1.659 |
R-factor | 0.1644 |
Rwork | 0.163 |
R-free | 0.18760 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.158 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.690 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.090 | 0.521 |
Number of reflections | 43097 | |
<I/σ(I)> | 22.6 | 2 |
Completeness [%] | 99.5 | 97.3 |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M calcium acetate, 0.1M MES:NaCl, 20% (w/v) PEG8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |