4R65
Ternary complex crystal structure of R258A mutant of DNA polymerase Beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-26 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5408 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.810, 79.940, 55.480 |
| Unit cell angles | 90.00, 107.30, 90.00 |
Refinement procedure
| Resolution | 20.736 - 1.950 |
| R-factor | 0.1905 |
| Rwork | 0.187 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.147 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.075 | 0.041 | 0.392 |
| Number of reflections | 28340 | ||
| <I/σ(I)> | 17.4 | ||
| Completeness [%] | 91.6 | 96.7 | 63.7 |
| Redundancy | 3.4 | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 16-18% PEG3350, 50 mM Imidazole, 350 mM Sodium Acetate, pH 7.5, VAPOR DIFFUSION, temperature 291K |
