4R5Z
Crystal structure of Rv3772 encoded aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 256.920, 77.556, 117.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.410 - 1.950 |
| R-factor | 0.18012 |
| Rwork | 0.179 |
| R-free | 0.20262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ffh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 167040 | |
| Completeness [%] | 97.0 | 93.6 |
| Redundancy | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1M succinic acid, 0.1M HEPES, 1% PEG 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
