4R5Q
Crystal structure and nuclease activity of the CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis containing a [2Fe-2S] cluster
Replaces: 4ONBExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 117.836, 117.836, 89.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.260 - 2.650 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.632 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.260 |
| High resolution limit [Å] | 2.650 |
| Rmerge | 0.047 |
| Number of reflections | 9378 |
| <I/σ(I)> | 41 |
| Completeness [%] | 99.4 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 3.5M Sodium FORMATE, 0.1M BIS-TRIS PROPANE, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
