4R4B
Crystal structure of the anti-hiv-1 antibody 2.2c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.011, 133.559, 90.774 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 48.662 - 2.199 |
| R-factor | 0.1679 |
| Rwork | 0.166 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u6a |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.897 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.199 | 2.200 |
| Number of reflections | 110015 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 93.6 | 67.8 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 100 MM NAOAC PH 4.5, 0-2 % V/V ISOPROPANOL, 2 M LI2SO4, 100 MM MGSO4, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
