4R3Z

Crystal structure of human ArgRS-GlnRS-AIMP1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	102.645, 313.253, 161.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.916 - 4.033
R-factor	0.2321
Rwork	0.229
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.961
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1745))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.920
High resolution limit [Å]	3.850	3.850
Number of reflections	20581
Completeness [%]	99.5	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	5-10% Methyl-pentene-Diol, 0.1M tris-HCl(pH7.4), 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K