4R3V
Structure of karilysin propeptide and catalytic MMP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.180, 121.690, 41.880 |
| Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
| Resolution | 60.800 - 2.010 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4in9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.800 | 2.110 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.105 | 0.105 |
| Number of reflections | 22975 | |
| <I/σ(I)> | 10.3 | 10.3 |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Bis-Tris propane, 0.2 M potassium thiocyanate, 20% (w/v) polyethylene glycol 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
