4R2S
Wilms Tumor Protein (WT1) Q369P zinc fingers in complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.148, 66.575, 36.073 |
| Unit cell angles | 90.00, 93.32, 90.00 |
Refinement procedure
| Resolution | 29.361 - 2.489 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2e |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.709 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.580 |
| High resolution limit [Å] | 2.489 | 2.490 |
| Rmerge | 0.087 | 0.250 |
| Number of reflections | 5690 | |
| <I/σ(I)> | 19.9 | 6.6 |
| Completeness [%] | 98.5 | 92.1 |
| Redundancy | 7.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 12% (w/v) PEG 3350, 0.1 M Ammonium Citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
