4R10

A conserved phosphorylation switch controls the interaction between cadherin and beta-catenin in vitro and in vivo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 43
Unit cell lengths	84.720, 84.720, 136.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.469 - 2.300
R-factor	0.1775
Rwork	0.175
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.721
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.046	0.019	0.421
Number of reflections	42695
<I/σ(I)>	14.5
Completeness [%]	99.6	99.5	99.3
Redundancy	3.9	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.2M sodium iodide, 17% PEG 3350, 0.1M Bis-Tris-Propane, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K