4QZI
Mouse Tdt, F401A mutant, in complex with a DSB substrate and Zn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972500 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.700, 71.523, 113.035 |
Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
Resolution | 22.420 - 2.650 |
R-factor | 0.1862 |
Rwork | 0.185 |
R-free | 0.20250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i2a |
RMSD bond length | 0.010 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.063 | 0.795 |
Number of reflections | 13390 | |
<I/σ(I)> | 13.6 | 2.1 |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |