4QXW
Crystal structure of the human CEACAM1 membrane distal amino terminal (N)-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.07810 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 107.160, 107.160, 61.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.923 - 2.040 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.097 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.1.26) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.923 | 2.100 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.198 | 0.010 |
| Total number of observations | 16250 | |
| Number of reflections | 23379 | |
| <I/σ(I)> | 8.8 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 50 mM TrisHCL buffer 7.5, 60% Tascimate 8.0, 1% Beta-octyl glucoside, 12% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
