4QXF

crystal structure of human LGR4 and Rspo1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2013-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97925
Spacegroup name	P 1 21 1
Unit cell lengths	44.867, 137.913, 82.560
Unit cell angles	90.00, 100.13, 90.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.21474
Rwork	0.213
R-free	0.24268
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qxe
RMSD bond length	0.009
RMSD bond angle	1.267
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.250	4.800	2.250
Rmerge	0.090
Number of reflections	43609
Completeness [%]	98.5	96.1	98.3
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1M MES pH 6.5, 0.2M sodium thiocyanate, 22% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K