4QQT

Crystal Structure of FGF Receptor (FGFR) 4 Tyrosine Kinase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-10
Detector	MAR CCD 165 mm
Wavelength(s)	0.97877
Spacegroup name	P 1 21 1
Unit cell lengths	42.384, 61.336, 61.084
Unit cell angles	90.00, 99.01, 90.00

Refinement procedure

Resolution	21.506 - 1.501
R-factor	0.2227
Rwork	0.222
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2psq
RMSD bond length	0.005
RMSD bond angle	1.042
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Rmerge	0.058
Number of reflections	49375
<I/σ(I)>	53.6
Completeness [%]	100.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.1 M MES, 20% (w/v) PEG 4000, 0.2 M Li2SO4 and 0.01 M Taurine, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K