4QQ4
CW-type zinc finger of MORC3 in complex with the amino terminus of histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791521 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.622, 64.860, 36.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.050 - 1.750 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o62 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.050 | 45.050 | 1.780 |
| High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
| Rmerge | 0.123 | 0.047 | 0.958 |
| Total number of observations | 692 | 5929 | |
| Number of reflections | 15427 | ||
| <I/σ(I)> | 12.5 | 28.4 | 2.3 |
| Completeness [%] | 100.0 | 98.2 | 99.9 |
| Redundancy | 6.9 | 4.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG-3350, 0.2 M ammonium chloride, vapor diffusion, sitting drop, temperature 293K |
