4QPD

Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (wild-type) complex with tetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	110
Detector technology	CCD
Collection date	2010-06-24
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.501, 100.415, 122.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.373 - 2.100
R-factor	0.1876
Rwork	0.185
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.050
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	39592
Completeness [%]	100.0	89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1~0.2M Bis-Tris, pH 5.5, 25~29%(w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K