4QNK
The structure of wt A. thaliana IGPD2 in complex with Mn2+ and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9507 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 225.130, 225.130, 225.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.980 - 1.750 |
| R-factor | 0.13154 |
| Rwork | 0.129 |
| R-free | 0.17949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.532 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.190 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.118 | 0.579 |
| Number of reflections | 187811 | |
| <I/σ(I)> | 11.7 | 2.2 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 290 | 0.1 M Bicine, pH 9.0, 10% w/v PEG6000, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
