4QN9
Structure of human NAPE-PLD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 95.099, 95.099, 444.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 82.358 - 2.652 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25332 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.387 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.358 | 2.720 |
| High resolution limit [Å] | 2.652 | 2.652 |
| Rmerge | 0.050 | 0.550 |
| Number of reflections | 35841 | |
| <I/σ(I)> | 26.7 | 3.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.1 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.9 M lithium sulfate, 0.1 M HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
