4QMD

Crystal structure of human envoplakin plakin repeat domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-16
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5417
Spacegroup name	P 21 21 21
Unit cell lengths	42.480, 68.950, 112.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.974 - 1.601
R-factor	0.172
Rwork	0.168
R-free	0.20460
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.018
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.974	4.680	1.700
High resolution limit [Å]	1.600	3.360	1.600
Rmerge	0.058	0.031	0.385
Number of reflections		5740	9413
<I/σ(I)>	27.9	95.66	2.09
Completeness [%]	92.9	100	67.2
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	20% PEG 3350, 0.1M BIS Tris-CL, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K