4QM7

Structure of bacterial polynucleotide kinase bound to GTP and pDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	130
Detector technology	PIXEL
Collection date	2013-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	46.170, 67.210, 118.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.085 - 1.800
R-factor	0.1649
Rwork	0.163
R-free	0.18820
Structure solution method	molecular refinement
RMSD bond length	0.014
RMSD bond angle	1.526
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.100	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	34969
Completeness [%]	99.7	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100 mM sodium citrate, 100 mM magnesium chloride, 18-24% PEG 6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K