4QL8
Crystal structure of Androgen Receptor in complex with the ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.129, 65.476, 68.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 2.100 |
| R-factor | 0.24522 |
| Rwork | 0.244 |
| R-free | 0.27502 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z95 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.784 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.134 |
| Number of reflections | 14439 | |
| <I/σ(I)> | 19.3 | 4.5 |
| Completeness [%] | 96.8 | 80.4 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES pH (6.5-8.5), 1.5M MgSO4 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
