4QKN
Crystal structure of FTO bound to a selective inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9735 |
| Spacegroup name | H 3 |
| Unit cell lengths | 141.260, 141.260, 83.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.860 - 2.200 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.208 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 37797 | |
| Completeness [%] | 98.9 | 97.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 100mM sodium citrate, 11.5% PEG3350, 8% isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
