4QKJ
Glycosylated form of human LLT1, a ligand for NKR-P1, in this structure forming hexamers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 70.060, 70.060, 101.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.000 - 2.750 |
| R-factor | 0.23022 |
| Rwork | 0.221 |
| R-free | 0.29780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hup |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.967 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.093 | 0.757 |
| Number of reflections | 4431 | |
| <I/σ(I)> | 16.2 | 2 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 288 | 40%(v/v) PEG300 and 0.1M citrate phosphate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
