4QJV

The X-ray crystal structure of Rpo3/Rpo11 heterodimer of euryarchaeal RNA polymerase from Thermococcus kodakarensis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785, 0.9788, 0.9600
Spacegroup name	P 21 21 21
Unit cell lengths	76.598, 88.169, 102.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.084 - 1.601
R-factor	0.1799
Rwork	0.179
R-free	0.21080
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.095
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	PHENIX ((phenix.refine: dev_1626))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	91780
<I/σ(I)>		3.6
Completeness [%]	99.1	99.1
Redundancy		5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch crystallization	10	277	0.1M CAPS (pH 10), 0.1M ammonium dihydrogen phosphate, 34%(w/v) PEG4000, Microbatch crystallization, temperature 277K