4QHO
Crystal structure of the human fat mass and obesity associated protein (FTO) in complex with CCO10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.2716 |
Spacegroup name | H 3 |
Unit cell lengths | 141.384, 141.384, 83.954 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.480 - 2.370 |
Rwork | 0.177 |
R-free | 0.20760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 4IE5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.216 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.400 |
High resolution limit [Å] | 2.320 | 5.000 | 2.320 |
Rmerge | 0.204 | 0.192 | |
Number of reflections | 26707 | ||
<I/σ(I)> | 11.5 | 2 | 12 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 7.2 | 8.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 100mM trisodium citrate, 8% PEG 3350, 4% tert-butanol, 8mg/ml protein, 1mM ZnSO4, 1mM inhibitor, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |