4QDH

Crystal Structure of the C-terminal Domain of Mouse TLR9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979390
Spacegroup name	P 31 2 1
Unit cell lengths	106.025, 106.025, 221.549
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.819 - 2.399
R-factor	0.2161
Rwork	0.214
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w3g
RMSD bond length	0.009
RMSD bond angle	1.501
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.820	2.470
High resolution limit [Å]	2.399	2.399
Number of reflections	57046
<I/σ(I)>	6.8	1.7
Completeness [%]		99.4
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.77	295	100 mM sodium citrate, 1.5 M ammonium sulfate, pH 4.77, VAPOR DIFFUSION, SITTING DROP, temperature 295K