4QCB
Protein-DNA complex of Vaccinia virus D4 with double-stranded non-specific DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.389, 92.327, 142.859 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.500 - 2.890 |
| R-factor | 0.21826 |
| Rwork | 0.216 |
| R-free | 0.26520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dof CHAIN A |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.500 | 3.050 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.158 | 0.505 |
| Number of reflections | 11991 | |
| <I/σ(I)> | 6.1 | 2.3 |
| Completeness [%] | 97.9 | 99.3 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2:1 protein solution/reservoir (16% PEG6000, 0.08 M Tris, pH 8.5, 20% glycerol), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
