4Q2Z
Fab fragment of HIV vaccine-elicited CD4bs-directed antibody, GE356, from a non-human primate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.243, 67.909, 69.980 |
| Unit cell angles | 92.39, 94.13, 109.69 |
Refinement procedure
| Resolution | 36.991 - 1.930 |
| R-factor | 0.2343 |
| Rwork | 0.232 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ktd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.119 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.620 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.549 | |
| Number of reflections | 60025 | |
| <I/σ(I)> | 19.2 | 1.1 |
| Completeness [%] | 95.0 | 95.8 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M Tri-lithium citrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
